1) demonstrate that RPL554 activates CFTR;
2) establish whether RPL554 can activate CFTR in R117H cells;
3) establish whether RPL554 can stimulate cilliary beat frequency in primary airway epithelial cells;
4) evaluate te role of the actin cystoskeleton using electrophysiology and fluorescence imaging and effect of RPL554 on spontaneous IL-8 release from CF airway epithelial cells

UK CF Trust Grant Code: Venture and Innovation Award 010