Project name: Project category: Kind of research: Country: Principle investigator:

Evaluation of the efficacy of novel antibacterial therapies for Pseudomonas aeruginosa biofilm eradication from airways of CF patients by using the ex vivo sputum biofilm model (EVSM)

New Therapies
Microbiology
Clinical research Belgium Prof. Dr. Mario Vaneechoutte, Prof. Dr. Frans De Baets
Start date: 01/10/2014
End date: 31/03/2016
Status: Finished
Abstract:

Many new compounds, which are claimed to enable eradication of biofilms, are being described. Because biofilms are also at the basis of chronic colonization by Pseudomonas aeruginosa of cystic fibrosis (CF) patients’ airways, these developments might render eradication of chronical infection of CF patients a realistic aim. However, these claims are made on the basis of artificial biofilm models, which for example lack the viscous sputum as it is present in the airways of CF patients. These in vitro results should therefore be confirmed by clinical trials, but these are cumbersome and expensive. Therefore, we suggest to test these compounds directly on sputum from chronically colonized patients. This ex vivo approach 1) is less expensive than setting up clinical trials, 2) enables to test many different compounds, and 3) has higher predictive power than artificial biofilms. It also 4) enables personalized medicine, enabling us to find the most promising strategies for each patient.

In summary, we propose to collect sputum from different patients, chronically infected with P. aeruginosa, and to test, directly on the sputum, the efficacy of new compounds to eradicate biofilms, in combination with existing antibiotic therapy. Killing of bacteria and destruction of biofilm will be analyzed by i) culture, ii) 3-D microscopy and iii) quantitation of P. aeruginosa (qPCR) after neutralizing DNA from killed bacteria with propidium monoazide (PMA-qPCR).

Researcher: Leen Van Simaey, Dr. Sabine Van Daele, Dr. Petra Schelstraete, Marleen Vanderkerken, Ann Raman, Linda Mahieu
Institute: University of Ghent
Department: Department of Microbiology - Laboratory Bacteriology Research (LBR), Faculty of Medicine & Health Sciences - MucoGent (CF Reference Center)
City: Gent
Project amount (€): 100.000

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